Detail Cantuman

Collection and Identification Of N Fixing Bacteria at Edamame Soybean’s
Field Center in Jember
Vivin Nur’Aini Fajrin
Study Program of Food Crop Production Technology
Majoring of Agricultural production
ABSTRACT
This research purposed to know n fixing bacteria at edamame soybean’s field Center in Jember regency. This research was conducted along 3 months from September 2016 until November 2016. The depth of a land activity had done in Jember subdistrict and the testing of N Fixing Bacteria that had done in Biosains Laboratory from State polytechnic of Jember. There were 2 factors with 6 treatments and 3 replication. The first factor was location factor (L) consisted of located in Jenggawah subdistrict (L1) and in Ajung subdistrict (L2) and the second factor were 3 levels of the depth land (k) consisted of 5 cm the depth of a land from a land surface (K1), 10 cm from a land surface (K2), 15cm from a land surface (K3). The data of this research analyzed as quantitative and qualitative. The quantitative data collected by counting the colony by TPC methode (Total Plate Count), and the qualitative data had gotten from observation of gram staining, observation of the speed of growth bacteria and observation of a pure bacteria. The result of this reasearch showed that bacteria which had isolated from vegetable soybean (Glycine max (L.) Merr.) was Rhizobium spp, it is bacteria type of N fixing bacteria, the result showed from observation of a pure bacteria was a pure white and pink after bred in Yema selection media+congo red.Rhizobium spp. which has the highest adaptation level is from Ajung District.It was taken at 15 cm below the ground surface. The adaptation level s 6,88x1030 CFU/gram of soil


Keywords : Jember subdistric Location, Rhizobium spp., The Depth of a Land,
Vegetable Soybean.